2013 ICORE Teachers "Omics" Workshop
(July
17 -19, 2013 in ICBR Education and Training Lab, Chen Lab and Polfer Lab)
Sponsored by the National Science
Foundation and the UF CPET program
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Introduction of the Proteomics
Lab by Ms. Drew Joseph from ICORE and Dr. Sixue Chen, Associate Professor
from Biology Department
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Dr. Chen introduces proteomics, plant materials and the objectives of
the workshop
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Sample preparation
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    Separation
of proteins extracted from pathogen-infected tomato leaves on SDS
polyacrylamide gels
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  Visualization
of gel images and excising protein bands for “fingerprinting”
identification
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Ms. Drew Joseph works with the teachers
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Mr. Jon Benskin, a high school teacher from Boca Raton Community High
School presents his proteomics lab protocol.
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Spotting protein digest on MALDI plate for peptide mass
fingerprinting
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Mr. Ning Zhu from the Chen lab shows how to fire the laser on a
MALDI-TOF mass spectrometer
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A lot of hands-on experiments including peptide mass fingerprint
acquisition and database searching to identify the unknown protein, cool
stuff.
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Dr. Cecilia Silva-Sanchez explains MALDI-TOF/TOF technology
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Dr. Sixue Chen demonstrates the nanoflow
liquid chromatography system
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Dr. Chen introduces the high-end LC-MS/MS system and demonstrates MS/MS
spectrum acquisition workflow
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Dr. Nick Polfer explains the Orbitrap technology
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The 2013 ICORE class
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Proteomics workshop is FUN!
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For more pictures, please click
here: http://www.cpet.ufl.edu/ICORE/ICORE2013/PhotoGallery.html
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