2012 ICORE Teachers "Omics" Workshop
(July 13 -14, 2012 in ICBR Education and Training Lab, Chen Lab and Polfer Lab)
Sponsored by the National Science Foundation and the UF CPET program
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ICORE 2011 Participants pictured in the front of the Emerging Pathogens Institute
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Introduction of the Proteomics Lab by Ms. Drew Joseph from ICORE and Dr. Sixue Chen, Associate Professor from Biology Department |

Dr. Chen introduces the plant materials and the objectives of the proteomics experiments
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Sample preparation
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Protein extraction from control tomato leaves and pathogen-infected tomato leaves |
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Dr. Chen chats with the teachers about sample preparation procedures
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Tong Zhang, a graduate student from Chen lab works with a teacher
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Lan Yang and Da Wang, two graduate students from Polfer lab work with the teachers
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An interactive lab |
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Michelle Chin, a high school student attends the workshop, Tong Zhang is helping with sample preparation
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Jennifer Parker, a graduate student from Chen lab talks with the teachers
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Drew and an undergraduate student work with the teachers
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Tong Zhang talks with a teacher
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Huizhuan Yan, a graduate student from Chen lab works with the teachers
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A lot of hands-on experiments |
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Dr. Chen gives a "omics" and systems biology lecture |
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Jennifer and Huizhuan demonstrate protein gel electrophoresis |
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Dr. Chen and Jennifer with the teachers |
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Loading the samples onto the gel
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Gel electrophoresis is COOL
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A little discussion about stuff |
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Drew amd Huizhuan help with the gel electrophoresis lab |
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Jennifer and Tong help with the gel electrophoresis lab |

How to take the gel out? |
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Take a good picture of the gel |

A nice separation of proteins, which band to cut? |
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Stain-free gels work really well
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Mass spectrometry lecture and demonstration by Drs. Cecilia Silva-Sanchez and Chen
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Protein in-gel digest spotting on MALDI sample plate |
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Acquiring and inspecting MALDI-TOF peptide fingerprints
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MALDI-TOF/TOF instrument used for the workshop
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Acquiring and analyzing more peptide mass fingerprints for protein identification |
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Dr. Chen introduces nanoflow LC-MS/MS
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Quadrupole ion trap and nanoflow HPLC technology
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LC-MS/MS data inspection |
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State-of-the-art Orbitrap technology |
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A lot of interactions |
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Proteomics workshop is FUN |
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